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WP1

WP1

WP1 – Assembling of barley collections, bioclimatic databases and phenotyping. (Leaders: Abderrahmane Djemel, ENSA; Khaled Soliman, ASUDG; other operational units: CSIC; CREA-GB; TEKFEN).

WP1 aims to gather suitable barley accessions and bioclimatic databases for studying barley adaptation and will organize common garden experiments along with phenotyping for stress resistance and phenological traits. WP1 will mine public and private germplasm collections of geo-referenced accessions maintained in Egypt, Italy, Spain, Tunisia, Turkey and Algeria, and other ex-situ collections (e.g. ICARDA germplasm collection), to identify a set of circa 1000 genotypes sampled along the gradients of Mediterranean climatic conditions, including temperature extremes. Agro-ecological zones and countries will be carefully sampled to ensure representativeness and create a permanent research tool. This population size will provide an unprecedented coverage of the whole Mediterranean basin and will ensure good resolution power for downstream analyses. In addition to these accessions, a subset of geo-referenced landraces used in connected projects (1.3.3) and sampled in countries of the Southern Mediterranean areas will be also included in the panel. WP1 will assemble a database of historical and current climatic series using publicly available resources and will compute relevant bioclimatic variables that characterize European and Mediterranean agro-ecological zones.

The whole panel of landraces will be multiplied for collecting tissues to extract DNA as well as for preparing enough seeds to phenotype these accessions for phenological traits in single rows. Based on results produced in WP2, WP1 will select subsets of accessions to a) examine the phenotypic differentiation of circa 80 landraces in common garden experiments (3-site-by-season combinations) using experimental sites differing with respect to particular bioclimatic factors; and b) will map new resistance genes via GWAS phenotyping circa 100 accessions at the seedling stage for drought tolerance and powdery mildew (~ 30 different isolates of Blumeria graminis) in controlled and field conditions.