English

WP2

WP2

WP2 Analyses of physiological and genetic adaptation to harsh Mediterranean conditions. (Leaders: Ernesto Igartua, CSIC; Agostino Fricano, CREA-GB; other operational units: UdL).

WP2 will characterize at the molecular level the panel of 1000 genotypes identified in WP1 using genotyping-by-sequencing methodology, and will examine the pattern of DNA variants to assess the population structure and genetic diversity. WP2 will develop a landscape genomics approach, combining genetic information with bioclimatic data of the sampling sites computed in WP1 to detect outlier loci that might underlie unknown adaptive traits using genome-environment association. WP2 will conduct whole genome re-sequencing at 15X of circa 80 barley landraces from the whole set of Mediterranean sub-populations and environments, including heat tolerant and intolerant genotypes identified in WP3. WP2 will use this sequencing data, population structure analysis, bioclimatic data and common garden results (WP1) to detect and validate genomic signatures of adaptation, such as sequences showing reduced genetic diversity and extended haplotype blocks. Moreover, WP2 will compute cross-population statistics to detect sequences with elevated genetic divergence measuring Fst, XtX, and comparing haplotype sizes. In addition to the genome-wide characterization, WP2 will genotype genes controlling phenological traits to identify relevant genetic variants. WP2 will interact with WP1 as landraces with exotic and new alleles will be phenotyped to estimate the effects of these genetic variants on barley phenology. Moreover, WP2 will study the performance of plants carrying different combinations of PpdH1 and HvVrn1 alleles in near isogenic lines (NILs) and subjected to artificially-induced long photoperiod in the field, after natural vernalization. Traits related to spike fertility that may be affected by these genes in absence of their effects on time to heading (due to the growing conditions making similar developmental patterns of sensitive and insensitive alleles) will be analyzed, including developmental processes (e.g. floret primordia initiation and degeneration) and crop-physiological parameters.